A novel near-infrared polymethine dye biosensor for rapid and selective detection of lithocholic acid.
Biosens Bioelectron
; 259: 116383, 2024 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-38749286
ABSTRACT
Lithocholic acid (LCA), a secondary bile acid, has emerged as a potential early diagnostic biomarker for various liver diseases. In this study, we introduce a novel near-infrared (NIR) polymethine dye-based biosensor, capable of sensitive and selective detection of LCA in phosphate buffer and artificial urine (AU) solutions. The detection mechanism relies on the formation of J-aggregates resulting from the interplay of 3,3-Diethylthiatricarbocyanine iodide (DiSC2(7)) dye molecules and LCA, which induces a distinctive red shift in both absorption and fluorescence spectra. The biosensor demonstrates a detection limit for LCA of 70 µM in PBS solution (pH 7.4), while in AU solution, it responds to an LCA concentration as low as â¼60 µM. Notably, the proposed biosensor exhibits outstanding selectivity for LCA, effectively distinguishing it from common interferents such as uric acid, ascorbic acid, and glucose. This rapid, straightforward, and cost-effective spectrometer-based method underscores its potential for early diagnosis of liver diseases by monitoring LCA concentrations.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Limite de Detecção
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Ácido Litocólico
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article