TRIM25 predominately associates with anti-viral stress granules.
Nat Commun
; 15(1): 4127, 2024 May 15.
Article
em En
| MEDLINE
| ID: mdl-38750080
ABSTRACT
Stress granules (SGs) are induced by various environmental stressors, resulting in their compositional and functional heterogeneity. SGs play a crucial role in the antiviral process, owing to their potent translational repressive effects and ability to trigger signal transduction; however, it is poorly understood how these antiviral SGs differ from SGs induced by other environmental stressors. Here we identify that TRIM25, a known driver of the ubiquitination-dependent antiviral innate immune response, is a potent and critical marker of the antiviral SGs. TRIM25 undergoes liquid-liquid phase separation (LLPS) and co-condenses with the SG core protein G3BP1 in a dsRNA-dependent manner. The co-condensation of TRIM25 and G3BP1 results in a significant enhancement of TRIM25's ubiquitination activity towards multiple antiviral proteins, which are mainly located in SGs. This co-condensation is critical in activating the RIG-I signaling pathway, thus restraining RNA virus infection. Our studies provide a conceptual framework for better understanding the heterogeneity of stress granule components and their response to distinct environmental stressors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por Vírus de RNA
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Ubiquitina-Proteína Ligases
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Proteínas com Motivo Tripartido
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Grânulos de Estresse
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article