Puerariae lobatae Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/ßcatenin signaling.
Mol Med Rep
; 30(1)2024 07.
Article
em En
| MEDLINE
| ID: mdl-38757304
ABSTRACT
Gut microbiota dysfunction is a key factor affecting chronic kidney disease (CKD) susceptibility. Puerariae lobatae Radix (PLR), a traditional Chinese medicine and food homologous herb, is known to promote the gut microbiota homeostasis; however, its role in renoprotection remains unknown. The present study aimed to investigate the efficacy and potential mechanism of PLR to alleviate CKD. An 8week 2% NaClfeeding murine model was applied to induce CKD and evaluate the therapeutic effect of PLR supplementary. After gavage for 8 weeks, The medium and high doses of PLR significantly alleviated CKDassociated creatinine, urine protein increasement and nephritic histopathological injury. Moreover, PLR protected kidney from fibrosis by reducing inflammatory response and downregulating the canonical Wnt/ßcatenin pathway. Furthermore, PLR rescued the gut microbiota dysbiosis and protected against high saltinduced gut barrier dysfunction. Enrichment of Akkermansia and Bifidobacterium was found after PLR intervention, the relative abundances of which were in positive correlation with normal maintenance of renal histology and function. Next, fecal microbiota transplantation experiment verified that the positive effect of PLR on CKD was, at least partially, exerted through gut microbiota reestablishment and downregulation of the Wnt/ßcatenin pathway. The present study provided evidence for a new function of PLR on kidney protection and put forward a potential therapeutic strategy target for CKD.
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Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
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Pueraria
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Insuficiência Renal Crônica
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Via de Sinalização Wnt
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Microbioma Gastrointestinal
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article