Diffusion-driven fed-batch fermentation in perforated ring flasks.
Biotechnol Lett
; 46(4): 571-582, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38758336
ABSTRACT
PURPOSE:
Simultaneous membrane-based feeding and monitoring of the oxygen transfer rate shall be introduced to the newly established perforated ring flask, which consists of a cylindrical glass flask with an additional perforated inner glass ring, for rapid bioprocess development.METHODS:
A 3D-printed adapter was constructed to enable monitoring of the oxygen transfer rate in the perforated ring flasks. Escherichia coli experiments in batch were performed to validate the adapter. Fed-batch experiments with different diffusion rates and feed solutions were performed.RESULTS:
The adapter and the performed experiments allowed a direct comparison of the perforated ring flasks with Erlenmeyer flasks. In batch cultivations, maximum oxygen transfer capacities of 80 mmol L-1 h-1 were reached with perforated ring flasks, corresponding to a 3.5 times higher capacity than in Erlenmeyer flasks. Fed-batch experiments with a feed reservoir concentration of 500 g glucose L-1 were successfully conducted. Based on the oxygen transfer rate, an ammonium limitation could be observed. By adding 40 g ammonium sulfate L-1 to the feed reservoir, the limitation could be prevented.CONCLUSION:
The membrane-based feeding, an online monitoring technique, and the perforated ring flask were successfully combined and offer a new and promising tool for screening and process development in biotechnology.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oxigênio
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Reatores Biológicos
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Escherichia coli
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Fermentação
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Técnicas de Cultura Celular por Lotes
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article