Probing the Surface Layer Modulation on Archaeal Mechanics and Adhesion at the Single-Cell Level.
Anal Chem
; 96(22): 8981-8989, 2024 06 04.
Article
em En
| MEDLINE
| ID: mdl-38758609
ABSTRACT
Addressing the challenge of understanding how cellular interfaces dictate the mechanical resilience and adhesion of archaeal cells, this study demonstrates the role of the surface layer (S-layer) in methanogenic archaea. Using a combination of atomic force microscopy and single-cell force spectroscopy, we quantified the impact of S-layer disruption on cell morphology, mechanical properties, and adhesion capabilities. We demonstrate that the S-layer is crucial for maintaining cell morphology, where its removal induces significant cellular enlargement and deformation. Mechanical stability of the cell surface is substantially compromised upon S-layer disruption, as evidenced by decreased Young's modulus values. Adhesion experiments revealed that the S-layer primarily facilitates hydrophobic interactions, which are significantly reduced after its removal, affecting both cell-cell and cell-bubble interactions. Our findings illuminate the S-layer's fundamental role in methanogen architecture and provide a chemical understanding of archaeal cell surfaces, with implications for enhancing methane production in biotechnological applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Propriedades de Superfície
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Microscopia de Força Atômica
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Análise de Célula Única
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article