Molecular insights into the prototypical single-stranded DNA-binding protein from E. coli.
Crit Rev Biochem Mol Biol
; 59(1-2): 99-127, 2024.
Article
em En
| MEDLINE
| ID: mdl-38770626
ABSTRACT
The SSB protein of Escherichia coli functions to bind single-stranded DNA wherever it occurs during DNA metabolism. Depending upon conditions, SSB occurs in several different binding modes. In the course of its function, SSB diffuses on ssDNA and transfers rapidly between different segments of ssDNA. SSB interacts with many other proteins involved in DNA metabolism, with 22 such SSB-interacting proteins, or SIPs, defined to date. These interactions chiefly involve the disordered and conserved C-terminal residues of SSB. When not bound to ssDNA, SSB can aggregate to form a phase-separated biomolecular condensate. Current understanding of the properties of SSB and the functional significance of its many intermolecular interactions are summarized in this review.
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Base de dados:
MEDLINE
Assunto principal:
DNA de Cadeia Simples
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Proteínas de Escherichia coli
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Proteínas de Ligação a DNA
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Escherichia coli
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article