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A data-driven single-cell and spatial transcriptomic map of the human prefrontal cortex.
Huuki-Myers, Louise A; Spangler, Abby; Eagles, Nicholas J; Montgomery, Kelsey D; Kwon, Sang Ho; Guo, Boyi; Grant-Peters, Melissa; Divecha, Heena R; Tippani, Madhavi; Sriworarat, Chaichontat; Nguyen, Annie B; Ravichandran, Prashanthi; Tran, Matthew N; Seyedian, Arta; Hyde, Thomas M; Kleinman, Joel E; Battle, Alexis; Page, Stephanie C; Ryten, Mina; Hicks, Stephanie C; Martinowich, Keri; Collado-Torres, Leonardo; Maynard, Kristen R.
Afiliação
  • Huuki-Myers LA; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Spangler A; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Eagles NJ; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Montgomery KD; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Kwon SH; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Guo B; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
  • Grant-Peters M; Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.
  • Divecha HR; Genetics and Genomic Medicine, Great Ormond Street Institute of Child Health, University College London, London WC1N 1EH, UK.
  • Tippani M; Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA.
  • Sriworarat C; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Nguyen AB; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Ravichandran P; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Tran MN; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
  • Seyedian A; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Hyde TM; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Kleinman JE; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Page SC; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Ryten M; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
  • Hicks SC; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
  • Martinowich K; Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
  • Collado-Torres L; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
  • Maynard KR; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21218, USA.
Science ; 384(6698): eadh1938, 2024 May 24.
Article em En | MEDLINE | ID: mdl-38781370
ABSTRACT
The molecular organization of the human neocortex historically has been studied in the context of its histological layers. However, emerging spatial transcriptomic technologies have enabled unbiased identification of transcriptionally defined spatial domains that move beyond classic cytoarchitecture. We used the Visium spatial gene expression platform to generate a data-driven molecular neuroanatomical atlas across the anterior-posterior axis of the human dorsolateral prefrontal cortex. Integration with paired single-nucleus RNA-sequencing data revealed distinct cell type compositions and cell-cell interactions across spatial domains. Using PsychENCODE and publicly available data, we mapped the enrichment of cell types and genes associated with neuropsychiatric disorders to discrete spatial domains.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Célula Única / Transcriptoma / Córtex Pré-Frontal Dorsolateral Limite: Adult / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Célula Única / Transcriptoma / Córtex Pré-Frontal Dorsolateral Limite: Adult / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article