Genetic Characterization of Lumpy Skin Disease Viruses Circulating in Lesotho Cattle.
Viruses
; 16(5)2024 05 11.
Article
em En
| MEDLINE
| ID: mdl-38793643
ABSTRACT
Lumpy skin disease is one of the fast-spreading viral diseases of cattle and buffalo that can potentially cause severe economic impact. Lesotho experienced LSD for the first time in 1947 and episodes of outbreaks occurred throughout the decades. In this study, eighteen specimens were collected from LSD-clinically diseased cattle between 2020 and 2022 from Mafeteng, Leribe, Maseru, Berea, and Mohales' Hoek districts of Lesotho. A total of 11 DNA samples were analyzed by PCR and sequencing of the extracellular enveloped virus (EEV) glycoprotein, G-protein-coupled chemokine receptor (GPCR), 30 kDa RNA polymerase subunit (RPO30), and B22R genes. All nucleotide sequences of the above-mentioned genes confirmed that the PCR amplicons of clinical samples are truly LSDV, as they were identical to respective LSDV isolates on the NCBI GenBank. Two of the elevem samples were further characterized by whole-genome sequencing. The analysis, based on both CaPV marker genes and complete genome sequences, revealed that the LSDV isolates from Lesotho cluster with the NW-like LSDVs, which includes the commonly circulating LSDV field isolates from Africa, the Middle East, the Balkans, Turkey, and Eastern Europe.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Doença Nodular Cutânea
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Vírus da Doença Nodular Cutânea
Limite:
Animals
País como assunto:
Africa
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article