A serotype-specific and tiled amplicon multiplex PCR method for whole genome sequencing of dengue virus.
J Virol Methods
; 328: 114968, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38796133
ABSTRACT
Dengue fever, a mosquito-borne viral disease of significant public health concern in tropical and subtropical regions, is caused by any of the four serotypes of the dengue virus (DENV1-4). Cutting-edge technologies like next-generation sequencing (NGS) are revolutionizing virology, enabling in-depth exploration of DENV's genetic diversity. Here, we present an optimized workflow for full-genome sequencing of DENV 1-4 utilizing tiled amplicon multiplex PCR and Illumina sequencing. Our assay, sequenced on the Illumina MiSeq platform, demonstrates its ability to recover the full-length dengue genome across various viral abundances in clinical specimens with high-quality base coverage. This high quality underscores its suitability for precise examination of intra-host diversity, enriching our understanding of viral evolution and holding potential for improved diagnostic and intervention strategies in regions facing dengue outbreaks.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genoma Viral
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Dengue
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Vírus da Dengue
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Sequenciamento de Nucleotídeos em Larga Escala
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Reação em Cadeia da Polimerase Multiplex
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Sorogrupo
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Sequenciamento Completo do Genoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article