Genotyping Zebrafish Point Mutant by Allele-Specific Blocking PCR.
Zebrafish
; 21(4): 297-299, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38808506
ABSTRACT
Genotyping zebrafish carrying wild-type, heterozygous, or homozygous copies of a mutant allele is often required for investigating gene specific functions, and is routinely performed to differentiate point mutants. In this study, we describe a modified allele-specific PCR method using an additional blocking primer to promote target sequence amplification while suppressing sequences with single mismatch. Using the tp53m214k point mutant as an example, we show that wild-type, heterozygous, and homozygous zebrafish can be easily distinguished using this simple PCR method, which could be widely adapted for genotyping zebrafish with point mutations or small nucleotide insertions/deletions.
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Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
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Reação em Cadeia da Polimerase
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Mutação Puntual
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Alelos
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Técnicas de Genotipagem
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article