Room-temperature serial synchrotron crystallography structure of Spinacia oleracea RuBisCO.
Acta Crystallogr F Struct Biol Commun
; 80(Pt 6): 117-124, 2024 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-38809540
ABSTRACT
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the enzyme responsible for the first step of carbon dioxide (CO2) fixation in plants, which proceeds via the carboxylation of ribulose 1,5-biphosphate. Because of the enormous importance of this reaction in agriculture and the environment, there is considerable interest in the mechanism of fixation of CO2 by RuBisCO. Here, a serial synchrotron crystallography structure of spinach RuBisCO is reported at 2.3â
Å resolution. This structure is consistent with earlier single-crystal X-ray structures of this enzyme and the results are a good starting point for a further push towards time-resolved serial synchrotron crystallography in order to better understand the mechanism of the reaction.
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Base de dados:
MEDLINE
Assunto principal:
Ribulose-Bifosfato Carboxilase
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Modelos Moleculares
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Síncrotrons
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Spinacia oleracea
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article