Induction of point and structural mutations in engineered yeast Saccharomyces cerevisiae improve carotenoid production.
World J Microbiol Biotechnol
; 40(7): 230, 2024 Jun 03.
Article
em En
| MEDLINE
| ID: mdl-38829459
ABSTRACT
ß-Carotene is an attractive compound and that its biotechnological production can be achieved by using engineered Saccharomyces cerevisiae. In a previous study, we developed a technique for the efficient establishment of diverse mutants through the introduction of point and structural mutations into the yeast genome. In this study, we aimed to improve ß-carotene production by applying this mutagenesis technique to S. cerevisiae strain that had been genetically engineered for ß-carotene production. Point and structural mutations were introduced into ß-carotene-producing engineered yeast. The resulting mutants showed higher ß-carotene production capacity than the parental strain. The top-performing mutant, HP100_74, produced 37.6 mg/L of ß-carotene, a value 1.9 times higher than that of the parental strain (20.1 mg/L). Gene expression analysis confirmed an increased expression of multiple genes in the glycolysis, mevalonate, and ß-carotene synthesis pathways. In contrast, expression of ERG9, which functions in the ergosterol pathway competing with ß-carotene production, was decreased in the mutant strain. The introduction of point and structural mutations represents a simple yet effective method for achieving mutagenesis in yeasts. This technique is expected to be widely applied in the future to produce chemicals via metabolic engineering of S. cerevisiae.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Beta Caroteno
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Proteínas de Saccharomyces cerevisiae
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Engenharia Metabólica
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article