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The impact of the European Society of Cardiology guidelines and whole exome sequencing on genetic testing in hereditary cardiac diseases.
Mio, Catia; Zucco, Jessica; Fabbro, Dora; Bregant, Elisa; Baldan, Federica; Allegri, Lorenzo; D'Elia, Angela Valentina; Collini, Valentino; Imazio, Massimo; Damante, Giuseppe; Faletra, Flavio.
Afiliação
  • Mio C; Department of Medicine (DMED), University of Udine, Udine, Italy.
  • Zucco J; Institute of Medical Genetics, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), Udine, Italy.
  • Fabbro D; Institute of Medical Genetics, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), Udine, Italy.
  • Bregant E; Institute of Medical Genetics, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), Udine, Italy.
  • Baldan F; Department of Medicine (DMED), University of Udine, Udine, Italy.
  • Allegri L; Department of Medicine (DMED), University of Udine, Udine, Italy.
  • D'Elia AV; Institute of Medical Genetics, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), Udine, Italy.
  • Collini V; Cardiology, Cardiothoracic Department, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), Udine, Italy.
  • Imazio M; Department of Medicine (DMED), University of Udine, Udine, Italy.
  • Damante G; Cardiology, Cardiothoracic Department, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), Udine, Italy.
  • Faletra F; Department of Medicine (DMED), University of Udine, Udine, Italy.
Clin Genet ; 106(4): 394-402, 2024 Oct.
Article em En | MEDLINE | ID: mdl-38837338
ABSTRACT
In the last decade, an incredible improvement has been made in elucidating the genetic bases of cardiomyopathies. Here we report the impact of either the European Society of Cardiology (ESC) guidelines or the use of whole exome sequencing (WES) in terms of a number of variants of uncertain significance (VUS) and missed diagnoses in a series of 260 patients affected by inherited cardiac disorders. Samples were analyzed using a targeted gene panel of 128 cardiac-related genes and/or WES in a subset of patients, with a three-tier approach. Analyzing (i) only a subset of genes related to the clinical presentation, strictly following the ESC guidelines, 20.77% positive test were assessed. The incremental diagnostic rate for (ii) the whole gene panel, and (iii) the WES was 4.71% and 11.67%, respectively. The diverse analytical approaches increased the number of VUSs and incidental findings. Indeed, the use of WES highlights that there is a small percentage of syndromic conditions that standard analysis would not have detected. Moreover, the use of targeted sequencing coupled with "narrow" analytical approach prevents the detection of variants in actionable genes that could allow for preventive treatment. Our data suggest that genetic testing might aid clinicians in the diagnosis of inheritable cardiac disorders.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Testes Genéticos / Sequenciamento do Exoma Limite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged País como assunto: Europa Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Testes Genéticos / Sequenciamento do Exoma Limite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged País como assunto: Europa Idioma: En Ano de publicação: 2024 Tipo de documento: Article