Molecular anchoring and fluorescent labeling in animals compatible with tissue clearing for 3D imaging.
Curr Opin Chem Biol
; 81: 102474, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38838505
ABSTRACT
Analyzing the quantity and distribution of molecules throughout intact biological tissue is crucial for understanding various biological phenomena. Traditional methods involving destructive extraction result in the loss of spatial information. Conversely, tissue-clearing techniques combined with fluorescence imaging have recently emerged as a powerful tool for deep tissue imaging without sacrificing spatial coverage. Key to this approach is the anchoring and labeling of targets in intact tissue. In this review, methods for anchoring and labeling proteins, lipids, carbohydrates, and small molecules are presented. Future directions include the development of activity-based probes that work in vivo and mark transient events with spatial information to enable a deeper understanding of biological phenomena.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imageamento Tridimensional
/
Corantes Fluorescentes
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article