Effects of glyphosate-based herbicide on gut microbes and hepatopancreatic metabolism in Pomacea canaliculata.
Ecotoxicol Environ Saf
; 280: 116549, 2024 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-38852467
ABSTRACT
Roundup®, a prominent glyphosate-based herbicide (GBH), holds a significant position in the global market. However, studies of its effects on aquatic invertebrates, including molluscs are limited. Pomacea canaliculata, a large freshwater snail naturally thrives in agricultural environments where GBH is extensively employed. Our investigation involved assessing the impact of two concentrations of GBH (at concentrations of 19.98â¯mg/L and 59.94â¯mg/L, corresponding to 6â¯mg/L and 18â¯mg/L glyphosate) during a 96â¯h exposure experiment on the intestinal bacterial composition and metabolites of P. canaliculata. Analysis of the 16â¯S rRNA gene demonstrated a notable reduction in the alpha diversity of intestinal bacteria due to GBH exposure. Higher GBH concentration caused a significant shift in the relative abundance of dominant bacteria, such as Bacteroides and Paludibacter. We employed widely-targeted metabolomics analysis to analyze alterations in the hepatopancreatic metabolic profile as a consequence of GBH exposure. The shifts in metabolites primarily affected lipid, amino acid, and glucose metabolism, resulting in compromised immune and adaptive capacities in P. canaliculata. These results suggested that exposure to varying GBH concentrations perpetuates adverse effects on intestinal and hepatopancreatic health of P. canaliculata. This study provides an understanding of the negative effects of GBH on P. canaliculata and may sheds light on its potential implications for other molluscs.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
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Hepatopâncreas
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Microbioma Gastrointestinal
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Glifosato
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Glicina
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Herbicidas
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article