RPS24 alternative splicing is a marker of cancer progression and epithelial-mesenchymal transition.
Sci Rep
; 14(1): 13246, 2024 06 10.
Article
em En
| MEDLINE
| ID: mdl-38853173
ABSTRACT
Although alternative splicing (AS) is a major mechanism that adds diversity to gene expression patterns, its precise role in generating variability in ribosomal proteins, known as ribosomal heterogeneity, remains unclear. The ribosomal protein S24 (RPS24) gene, encoding a ribosomal component, undergoes AS; however, in-depth studies have been challenging because of three microexons between exons 4 and 6. We conducted a detailed analysis of RPS24 AS isoforms using a direct approach to investigate the splicing junctions related to these microexons, focusing on four AS isoforms. Each of these isoforms showed tissue specificity and relative differences in expression among cancer types. Significant differences in the proportions of these RPS24 AS isoforms between cancerous and normal tissues across diverse cancer types were also observed. Our study highlighted a significant correlation between the expression levels of a specific RPS24 AS isoform and the epithelial-mesenchymal transition process in lung and breast cancers. Our research contributes to a better understanding of the intricate regulatory mechanisms governing AS of ribosomal protein genes and highlights the biological implications of RPS24 AS isoforms in tissue development and tumorigenesis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Ribossômicas
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Biomarcadores Tumorais
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Regulação Neoplásica da Expressão Gênica
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Processamento Alternativo
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Transição Epitelial-Mesenquimal
Limite:
Female
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Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article