The miR-30-5p/TIA-1 axis directs cellular senescence by regulating mitochondrial dynamics.
Cell Death Dis
; 15(6): 404, 2024 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-38858355
ABSTRACT
Senescent cells exhibit a diverse spectrum of changes in their morphology, proliferative capacity, senescence-associated secretory phenotype (SASP) production, and mitochondrial homeostasis. These cells often manifest with elongated mitochondria, a hallmark of cellular senescence. However, the precise regulatory mechanisms orchestrating this phenomenon remain predominantly unexplored. In this study, we provide compelling evidence for decreases in TIA-1, a pivotal regulator of mitochondrial dynamics, in models of both replicative senescence and ionizing radiation (IR)-induced senescence. The downregulation of TIA-1 was determined to trigger mitochondrial elongation and enhance the expression of senescence-associated ß-galactosidase, a marker of cellular senescence, in human foreskin fibroblast HS27 cells and human keratinocyte HaCaT cells. Conversely, the overexpression of TIA-1 mitigated IR-induced cellular senescence. Notably, we identified the miR-30-5p family as a novel factor regulating TIA-1 expression. Augmented expression of the miR-30-5p family was responsible for driving mitochondrial elongation and promoting cellular senescence in response to IR. Taken together, our findings underscore the significance of the miR-30-5p/TIA-1 axis in governing mitochondrial dynamics and cellular senescence.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Senescência Celular
/
MicroRNAs
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Dinâmica Mitocondrial
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Antígeno-1 Intracelular de Células T
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Mitocôndrias
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article