Circulating cell-free RNA in blood as a host response biomarker for detection of tuberculosis.
Nat Commun
; 15(1): 4949, 2024 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-38858368
ABSTRACT
Tuberculosis (TB) remains a leading cause of death from an infectious disease worldwide, partly due to a lack of effective strategies to screen and triage individuals with potential TB. Whole blood RNA signatures have been tested as biomarkers for TB, but have failed to meet the World Health Organization's (WHO) optimal target product profiles (TPP). Here, we use RNA sequencing and machine-learning to investigate the utility of plasma cell-free RNA (cfRNA) as a host-response biomarker for TB in cohorts from Uganda, Vietnam and Philippines. We report a 6-gene cfRNA signature, which differentiates TB-positive and TB-negative individuals with AUC = 0.95, 0.92, and 0.95 in test, training and validation, respectively. This signature meets WHO TPPs (sensitivity 97.1% [95% CI 80.9-100%], specificity 85.2% [95% CI 72.4-100%]) regardless of geographic location, sample collection method and HIV status. Overall, our results identify plasma cfRNA as a promising host response biomarker to diagnose TB.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Tuberculose
/
Biomarcadores
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Ácidos Nucleicos Livres
Limite:
Adult
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Female
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Humans
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Male
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Middle aged
País como assunto:
Africa
/
Asia
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article