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Maf1 phosphorylation is regulated through the action of prefoldin-like Bud27 on PP4 phosphatase in Saccharomyces cerevisiae.
Gutiérrez-Santiago, Francisco; Martínez-Fernández, Verónica; Garrido-Godino, Ana Isabel; Colino-Palomino, Cristina; Clemente-Blanco, Andrés; Conesa, Christine; Acker, Joël; Navarro, Francisco.
Afiliação
  • Gutiérrez-Santiago F; Departamento de Biología Experimental-Genética; Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071. Jaén, Spain.
  • Martínez-Fernández V; Departamento de Biología Experimental-Genética; Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071. Jaén, Spain.
  • Garrido-Godino AI; Departamento de Biología Experimental-Genética; Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071. Jaén, Spain.
  • Colino-Palomino C; Departamento de Biología Experimental-Genética; Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071. Jaén, Spain.
  • Clemente-Blanco A; Instituto de Biología Funcional y Genómica (IBFG), CSIC-USAL, Salamanca, Spain.
  • Conesa C; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, F-91191 Gif-sur-Yvette, France.
  • Acker J; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, F-91191 Gif-sur-Yvette, France.
  • Navarro F; Departamento de Biología Experimental-Genética; Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071. Jaén, Spain.
Nucleic Acids Res ; 52(12): 7081-7095, 2024 Jul 08.
Article em En | MEDLINE | ID: mdl-38864693
ABSTRACT
Bud27 is a prefoldin-like protein that participates in transcriptional regulation mediated by the three RNA polymerases in Saccharomyces cerevisiae. Lack of Bud27 significantly affects RNA pol III transcription, although the involved mechanisms have not been characterized. Here, we show that Bud27 regulates the phosphorylation state of the RNA pol III transcriptional repressor, Maf1, influences its nuclear localization, and likely its activity. We demonstrate that Bud27 is associated with the Maf1 main phosphatase PP4 in vivo, and that this interaction is required for proper Maf1 dephosphorylation. Lack of Bud27 decreases the interaction among PP4 and Maf1, Maf1 dephosphorylation, and its nuclear entry. Our data uncover a new nuclear function of Bud27, identify PP4 as a novel Bud27 interactor and demonstrate the effect of this prefoldin-like protein on the posttranslational regulation of Maf1. Finally, our data reveal a broader effect of Bud27 on PP4 activity by influencing, at least, the phosphorylation of Rad53.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Saccharomyces cerevisiae / Fosfoproteínas Fosfatases / Proteínas de Saccharomyces cerevisiae Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Saccharomyces cerevisiae / Fosfoproteínas Fosfatases / Proteínas de Saccharomyces cerevisiae Idioma: En Ano de publicação: 2024 Tipo de documento: Article