Arabidopsis cryptochromes interact with SOG1 to promote the repair of DNA double-strand breaks.
Biochem Biophys Res Commun
; 724: 150233, 2024 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-38865814
ABSTRACT
Cryptochromes (CRYs) are blue light (BL) photoreceptors to regulate a variety of physiological processes including DNA double-strand break (DSB) repair. SUPPRESSOR OF GAMMA RADIATION 1 (SOG1) acts as the central transcription factor of DNA damage response (DDR) to induce the transcription of downstream genes, including DSB repair-related genes BRCA1 and RAD51. Whether CRYs regulate DSB repair by directly modulating SOG1 is unknown. Here, we demonstrate that CRYs physically interact with SOG1. Disruption of CRYs and SOG1 leads to increased sensitivity to DSBs and reduced DSB repair-related genes' expression under BL. Moreover, we found that CRY1 enhances SOG1's transcription activation of DSB repair-related gene BRCA1. These results suggest that the mechanism by which CRYs promote DSB repair involves positive regulation of SOG1's transcription of its target genes, which is likely mediated by CRYs-SOG1 interaction.
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MEDLINE
Assunto principal:
Arabidopsis
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Proteínas de Arabidopsis
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Reparo do DNA
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Quebras de DNA de Cadeia Dupla
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Criptocromos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article