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Glyoxalase 1: Emerging biomarker and therapeutic target in cervical cancer progression.
Kim, Ji-Young; Jung, Ji-Hye; Jung, Soryung; Lee, Sanghyuk; Lee, Hyang Ah; Ouh, Yung-Taek; Hong, Seok-Ho.
Afiliação
  • Kim JY; Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea.
  • Jung JH; Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea.
  • Jung S; Department of Life Science, Ewha Womans University, Seoul, Republic of Korea.
  • Lee S; Department of Life Science, Ewha Womans University, Seoul, Republic of Korea.
  • Lee HA; Department of Obstetrics and Gynecology, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea.
  • Ouh YT; Department of Obstetrics and Gynecology, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea.
  • Hong SH; Department of Obstetrics and Gynecology, Ansan Hospital, Korea University College of Medicine, Gyeonggi, Republic of Korea.
PLoS One ; 19(6): e0299345, 2024.
Article em En | MEDLINE | ID: mdl-38870176
ABSTRACT

INTRODUCTION:

Cervical cancer presents a significant global health challenge, disproportionately impacting underserved populations with limited access to healthcare. Early detection and effective management are vital in addressing this public health concern. This study focuses on Glyoxalase-1 (GLO1), an enzyme crucial for methylglyoxal detoxification, in the context of cervical cancer.

METHODS:

We assessed GLO1 expression in cervical cancer patient samples using immunohistochemistry. In vitro experiments using HeLa cells were conducted to evaluate the impact of GLO1 inhibition on cell viability and migration. Single-cell RNA sequencing (scRNA-seq) and gene set variation analysis were utilized to investigate the role of GLO1 in the metabolism of cervical cancer. Additionally, public microarray data were analyzed to determine GLO1 expression across various stages of cervical cancer.

RESULTS:

Our analysis included 58 cervical cancer patients, and showed that GLO1 is significantly upregulated in cervical cancer tissues compared to normal cervical tissues, independent of pathological findings and disease stage. In vitro experiments indicated that GLO1 inhibition by S-p-bromobenzylglutathione cyclopentyl diester decreased cell viability and migration in cervical cancer cell lines. Analyses of scRNA-seq data and public gene expression datasets corroborated the overexpression of GLO1 and its involvement in cancer metabolism, particularly glycolysis. An examination of expression data from precancerous lesions revealed a progressive increase in GLO1 expression from normal tissue to invasive cervical cancer.

CONCLUSIONS:

This study highlights the critical role of GLO1 in the progression of cervical cancer, presenting it as a potential biomarker and therapeutic target. These findings contribute valuable insights towards personalized treatment approaches and augment the ongoing efforts to combat cervical cancer. Further research is necessary to comprehensively explore GLO1's potential in clinical applications.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Neoplasias do Colo do Útero / Lactoilglutationa Liase Limite: Adult / Female / Humans / Middle aged Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Neoplasias do Colo do Útero / Lactoilglutationa Liase Limite: Adult / Female / Humans / Middle aged Idioma: En Ano de publicação: 2024 Tipo de documento: Article