A near-infrared lysosomal probe for dynamic sulfur dioxide monitoring in inflammation.
Spectrochim Acta A Mol Biomol Spectrosc
; 320: 124647, 2024 Nov 05.
Article
em En
| MEDLINE
| ID: mdl-38880075
ABSTRACT
Inflammation is a complex physiological response involving various cellular and molecular events. Sulfur dioxide (SO2), recognized as both an endogenous signaling molecule and anti-inflammatory agent, plays a crucial role in modulating inflammation and maintaining cellular homeostasis. To gain deeper insights into the dynamics of inflammation-related processes, real-time monitoring of SO2 concentrations within cellular organelles is imperative. Here, we developed a near-infrared fluorescent probe, R2, equipped with lysosomal targeting features. R2 effectively monitors dynamic SO2 concentration changes during inflammation. The fluorescence intensity at 703 nm of R2 shows a strong linear correlation with the concentration of SO2, displaying a rapid response time to SO2 within 10 s and maintaining excellent photostability. The successful application of R2 in elucidating dynamic SO2 concentration changes in lysosomal during cellular and rat inflammatory processes underscores its significant potential as a tool for understanding the pathogenesis of inflammation-related diseases.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Dióxido de Enxofre
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Corantes Fluorescentes
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Inflamação
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Lisossomos
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article