Dynamic Regulation of Cell Mechanotransduction through Sequentially Controlled Mobile Surfaces.
Nano Lett
; 24(26): 7953-7961, 2024 Jul 03.
Article
em En
| MEDLINE
| ID: mdl-38888317
ABSTRACT
The physical properties of nanoscale cell-extracellular matrix (ECM) ligands profoundly impact biological processes, such as adhesion, motility, and differentiation. While the mechanoresponse of cells to static ligands is well-studied, the effect of dynamic ligand presentation with "adaptive" properties on cell mechanotransduction remains less understood. Utilizing a controllable diffusible ligand interface, we demonstrated that cells on surfaces with rapid ligand mobility could recruit ligands through activating integrin α5ß1, leading to faster focal adhesion growth and spreading at the early adhesion stage. By leveraging UV-light-sensitive anchor molecules to trigger a "dynamic to static" transformation of ligands, we sequentially activated α5ß1 and αvß3 integrins, significantly promoting osteogenic differentiation of mesenchymal stem cells. This study illustrates how manipulating molecular dynamics can directly influence stem cell fate, suggesting the potential of "sequentially" controlled mobile surfaces as adaptable platforms for engineering smart biomaterial coatings.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Propriedades de Superfície
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Adesão Celular
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Diferenciação Celular
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Mecanotransdução Celular
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Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article