Bioluminescent aptamer-based microassay for detection of melanoma inhibitory activity protein (MIA).
Anal Methods
; 16(26): 4234-4239, 2024 Jul 04.
Article
em En
| MEDLINE
| ID: mdl-38899488
ABSTRACT
Melanoma inhibitory activity protein (MIA) does obviously offer the potential to reveal clinical manifestations of melanoma. Despite a pressing need for effective diagnosis of this highly fatal disease, there are no clinically approved MIA detection ELISA kits available. A recommended MIA threshold has not yet been defined, mostly by reason of variability in immunoglobulins' affinity and stability, the difference in sample preparation and assay conditions. Here we present a pair of high-affinity DNA aptamers developed as an alternative recognition and binding element for MIA detection. Their stability and reproducible synthesis are expected to ensure this analysis under standard conditions. The devised aptamer-based solid-phase microassay of model standard and control human sera involves luciferase NLuc as a highly sensitive reporter. Bioluminescence dependence on MIA concentration ranges in a linear manner from 2.5 to 250 ng mL-1, providing a MIA detection limit of 1.67 ± 0.57 ng mL-1.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Aptâmeros de Nucleotídeos
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Medições Luminescentes
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Melanoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article