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Protocol for the hyperinsulinemic euglycemic clamp to measure glucose kinetics in rats.
Pereira, Sandra; Hahn, Margaret K; Humber, Bailey; Chaudhry, Talha; Wu, Sally; Agarwal, Sri Mahavir; Dimitrova, Nanka; Giacca, Adria.
Afiliação
  • Pereira S; Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Department of Physiology, University of Toronto, Toronto, ON, Canada. Electronic address: sandra.pereira@camh.ca.
  • Hahn MK; Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada; Department of Psychiatry, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada; Department of
  • Humber B; Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada.
  • Chaudhry T; Department of Physiology, University of Toronto, Toronto, ON, Canada.
  • Wu S; Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada.
  • Agarwal SM; Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Department of Psychiatry, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada.
  • Dimitrova N; Department of Comparative Medicine, University of Toronto, Toronto, ON, Canada.
  • Giacca A; Department of Physiology, University of Toronto, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada. Electronic address: adria.giacca@utoronto.ca.
STAR Protoc ; 5(3): 103143, 2024 Jun 19.
Article em En | MEDLINE | ID: mdl-38900633
ABSTRACT
In rats, cannulation of the jugular vein and the carotid artery precedes the use of the hyperinsulinemic euglycemic clamp to determine insulin sensitivity in vivo. Here, we present a vascular surgery protocol to allow the infusion of substances via the vein and the collection of blood samples from the artery on the day of the hyperinsulinemic euglycemic clamp. We describe steps for preparing for and performing catheterization surgery. We then detail procedures for clamp preparation and its use. For complete details on the use and execution of this protocol, please refer to Pereira et al.1,2,3.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article