Bayesian-frequentist hybrid inference framework for single cell RNA-seq analyses.
Hum Genomics
; 18(1): 69, 2024 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-38902839
ABSTRACT
BACKGROUND:
Single cell RNA sequencing technology (scRNA-seq) has been proven useful in understanding cell-specific disease mechanisms. However, identifying genes of interest remains a key challenge. Pseudo-bulk methods that pool scRNA-seq counts in the same biological replicates have been commonly used to identify differentially expressed genes. However, such methods may lack power due to the limited sample size of scRNA-seq datasets, which can be prohibitively expensive.RESULTS:
Motivated by this, we proposed to use the Bayesian-frequentist hybrid (BFH) framework to increase the power and we showed in simulated scenario, the proposed BFH would be an optimal method when compared with other popular single cell differential expression methods if both FDR and power were considered. As an example, the method was applied to an idiopathic pulmonary fibrosis (IPF) case study.CONCLUSION:
In our IPF example, we demonstrated that with a proper informative prior, the BFH approach identified more genes of interest. Furthermore, these genes were reasonable based on the current knowledge of IPF. Thus, the BFH offers a unique and flexible framework for future scRNA-seq analyses.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Teorema de Bayes
/
Análise de Sequência de RNA
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Análise de Célula Única
/
RNA-Seq
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article