Profiling Mouse Brain Single-Cell-Type Proteomes Via Adeno-Associated Virus-Mediated Proximity Labeling and Mass Spectrometry.

Shrestha, Him K; Sun, Huan; Wang, Ju; Peng, Junmin

Shrestha, Him K; Sun, Huan; Wang, Ju; Peng, Junmin.

Afiliação

Shrestha HK; Department of Structural Biology, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Sun H; Department of Structural Biology, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Wang J; Department of Structural Biology, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Peng J; Department of Structural Biology, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA. junmin.peng@stjude.org.

Methods Mol Biol ; 2817: 115-132, 2024.

Article em En | MEDLINE | ID: mdl-38907151

Assuntos

Encéfalo; Dependovirus; Proteoma; Proteômica; Espectrometria de Massas em Tandem; Dependovirus/genética; Dependovirus/metabolismo; Animais; Camundongos; Proteômica/métodos; Proteoma/análise; Encéfalo/metabolismo; Espectrometria de Massas em Tandem/métodos; Análise de Célula Única/métodos; Neurônios/metabolismo; Cromatografia Líquida/métodos; Vetores Genéticos/genética; Biotinilação; Espectrometria de Massas/métodos; Astrócitos/metabolismo

Palavras-chave

Adeno-associated viruses (AAVs); Astrocyte; BioID; Biotin; Liquid chromatography; Mass spectrometry; Neuron; Proteome; Proximity labeling; Single-cell-type proteomics; Tandem mass tag; TurboID

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Encéfalo / Dependovirus / Proteoma / Proteômica / Espectrometria de Massas em Tandem Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

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