The catalytic activity of methyltransferase METTL15 is dispensable for its role in mitochondrial ribosome biogenesis.
RNA Biol
; 21(1): 23-30, 2024 Jan.
Article
em En
| MEDLINE
| ID: mdl-38913872
ABSTRACT
Ribosomes are large macromolecular complexes composed of both proteins and RNA, that require a plethora of factors and post-transcriptional modifications for their biogenesis. In human mitochondria, the ribosomal RNA is post-transcriptionally modified at ten sites. The N4-methylcytidine (m4C) methyltransferase, METTL15, modifies the 12S rRNA of the small subunit at position C1486. The enzyme is essential for mitochondrial protein synthesis and assembly of the mitoribosome small subunit, as shown here and by previous studies. Here, we demonstrate that the m4C modification is not required for small subunit biogenesis, indicating that the chaperone-like activity of the METTL15 protein itself is an essential component for mitoribosome biogenesis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA Ribossômico
/
Ribossomos Mitocondriais
/
Metiltransferases
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article