Tracking lipid synthesis using 2H2O and 2H-NMR spectroscopy in black soldier fly (Hermetia illucens) larvae fed with macroalgae.
J Exp Biol
; 227(12)2024 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-38916067
ABSTRACT
Black soldier fly (Hermetia illucens) larvae are used to upcycle biowaste into insect biomass for animal feed. Previous research on black soldier fly has explored the assimilation of dietary fatty acids (FAs), but endogenous FA synthesis and modification remain comparatively unexplored. This study presents a 1H/2H-NMR methodology for measuring lipid synthesis in black soldier fly larvae using diluted deuterated water (2H2O) as a stable isotopic tracer delivered through the feeding media. This approach was validated by measuring 2H incorporation into the larvae's body water and consequent labelling of FA esterified into triacylglycerols. A 5% 2H enrichment in the body water, adequate to label the FA, is achieved after 24â
h in a substrate with 10% 2H2O. A standard feeding trial using an invasive macroalgae was designed to test this method, revealing de novo lipogenesis was lower in larvae fed with macroalgae, probably related to the poor nutritional value of the diet.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Alga Marinha
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Espectroscopia de Ressonância Magnética
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Óxido de Deutério
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Larva
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article