The primitive endoderm supports lineage plasticity to enable regulative development.
Cell
; 187(15): 4010-4029.e16, 2024 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-38917790
ABSTRACT
Mammalian blastocyst formation involves the specification of the trophectoderm followed by the differentiation of the inner cell mass into embryonic epiblast and extra-embryonic primitive endoderm (PrE). During this time, the embryo maintains a window of plasticity and can redirect its cellular fate when challenged experimentally. In this context, we found that the PrE alone was sufficient to regenerate a complete blastocyst and continue post-implantation development. We identify an in vitro population similar to the early PrE in vivo that exhibits the same embryonic and extra-embryonic potency and can form complete stem cell-based embryo models, termed blastoids. Commitment in the PrE is suppressed by JAK/STAT signaling, collaborating with OCT4 and the sustained expression of a subset of pluripotency-related transcription factors that safeguard an enhancer landscape permissive for multi-lineage differentiation. Our observations support the notion that transcription factor persistence underlies plasticity in regulative development and highlight the importance of the PrE in perturbed development.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Blastocisto
/
Diferenciação Celular
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Endoderma
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article