Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor.
Methods Mol Biol
; 2810: 75-83, 2024.
Article
em En
| MEDLINE
| ID: mdl-38926273
ABSTRACT
Large culture volumes are often required when expression constructs are particularly low-yielding or when end uses require significant amounts of material. In these cases, a single homogeneous culture is usually more convenient, in terms of both consistency of expression and labor/resource requirements, than multiple parallel cultures. Using a WAVE Bioreactor culture, volumes as high as 500L may be achieved in a single vessel. Here, we describe the transfection of Expi293F cells in a disposable 50L Cellbag on a WAVE Bioreactor platform to produce recombinant protein. The methods described herein may be adapted, with suitable optimizations, for other suspension-adapted mammalian cell lines.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
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Transfecção
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Reatores Biológicos
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article