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Exploring the endocrine disrupting potential of a complex mixture of PAHs in the estrogen pathway in Oreochromis niloticus hepatocytes.
de Souza, Tugstênio Lima; da Luz, Jessica Zablocki; Roque, Aliciane de Almeida; Opuskevitch, Iracema; Ferreira, Fernando Cesar Alves da Silva; Ribeiro, Ciro Alberto de Oliveira; Neto, Francisco Filipak.
Afiliação
  • de Souza TL; Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, CEP 81.531-980, Curitiba, PR, Brazil. Electronic address: tugstenio@ufpr.br.
  • da Luz JZ; Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, CEP 81.531-980, Curitiba, PR, Brazil.
  • Roque AA; Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, CEP 81.531-980, Curitiba, PR, Brazil.
  • Opuskevitch I; Copel GeT-SOS/DNGT - Rua José Izidoro Biazetto, no. 18, Bloco A, CEP 81200-240, Curitiba, PR, Brazil.
  • Ferreira FCADS; Copel GeT-SOS/DNGT - Rua José Izidoro Biazetto, no. 18, Bloco A, CEP 81200-240, Curitiba, PR, Brazil.
  • Ribeiro CAO; Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, CEP 81.531-980, Curitiba, PR, Brazil.
  • Neto FF; Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, CEP 81.531-980, Curitiba, PR, Brazil. Electronic address: filipak@ufpr.br.
Aquat Toxicol ; 273: 107002, 2024 Aug.
Article em En | MEDLINE | ID: mdl-38936242
ABSTRACT
This study aimed to investigate the toxicity and endocrine disrupting potential of a complex mixture of polycyclic aromatic hydrocarbons (PAHs) in the estrogen pathway using hepatocytes of Nile tilapia Oreochromis niloticus, the hepatocytes were exposed to various concentrations of the PAH mixture, and multiple endpoints were evaluated to assess their effects on cell viability, gene expression, oxidative stress markers, and efflux activity. The results revealed that the PAH mixture had limited effects on hepatocyte metabolism and cell adhesion, as indicated by the non-significant changes observed in MTT metabolism, neutral red retention, and crystal violet staining. However, significant alterations were observed in the expression of genes related to the estrogen pathway. Specifically, vitellogenin (vtg) exhibited a substantial increase of approximately 120% compared to the control group. Similarly, estrogen receptor 2 (esr2) showed a significant upregulation of approximately 90%. In contrast, no significant differences were observed in the expression of estrogen receptor 1 (esr1) and the G protein-coupled estrogen receptor 1 (gper1). Furthermore, the PAH mixture elicited complex responses in oxidative stress markers. While reactive oxygen species (ROS) and reactive nitrogen species (RNS) levels remained unchanged, the activity of catalase (Cat) was significantly reduced, whereas superoxide dismutase (Sod) activity, glutathione S-transferase (Gst) activity, and non-protein thiols levels were significantly elevated. In addition, the PAH mixture significantly influenced efflux activity, as evidenced by the increased efflux of rhodamine and calcein, indicating alterations in multixenobiotic resistance (MXR)-associated proteins. Overall, these findings, associated with bioinformatic analysis, highlight the potential of the PAH mixture to modulate the estrogen pathway and induce oxidative stress in O. niloticus hepatocytes. Understanding the mechanisms underlying these effects is crucial for assessing the ecological risks of PAH exposure and developing appropriate strategies to mitigate their adverse impacts on aquatic organisms.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Hidrocarbonetos Policíclicos Aromáticos / Poluentes Químicos da Água / Estresse Oxidativo / Hepatócitos / Ciclídeos / Estrogênios / Disruptores Endócrinos Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Hidrocarbonetos Policíclicos Aromáticos / Poluentes Químicos da Água / Estresse Oxidativo / Hepatócitos / Ciclídeos / Estrogênios / Disruptores Endócrinos Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article