Nucleoporin Elys attaches peripheral chromatin to the nuclear pores in interphase nuclei.
Commun Biol
; 7(1): 783, 2024 Jun 29.
Article
em En
| MEDLINE
| ID: mdl-38951619
ABSTRACT
Transport of macromolecules through the nuclear envelope (NE) is mediated by nuclear pore complexes (NPCs) consisting of nucleoporins (Nups). Elys/Mel-28 is the Nup that binds and connects the decondensing chromatin with the reassembled NPCs at the end of mitosis. Whether Elys links chromatin with the NE during interphase is unknown. Here, using DamID-seq, we identified Elys binding sites in Drosophila late embryos and divided them into those associated with nucleoplasmic or with NPC-linked Elys. These Elys binding sites are located within active or inactive chromatin, respectively. Strikingly, Elys knockdown in S2 cells results in peripheral chromatin displacement from the NE, in decondensation of NE-attached chromatin, and in derepression of genes within. It also leads to slightly more compact active chromatin regions. Our findings indicate that NPC-linked Elys, together with the nuclear lamina, anchors peripheral chromatin to the NE, whereas nucleoplasmic Elys decompacts active chromatin.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Poro Nuclear
/
Complexo de Proteínas Formadoras de Poros Nucleares
/
Proteínas de Drosophila
/
Interfase
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article