De Novo Long-Read Genome Assembly and Annotation of the Luna Moth (Actias luna) Fully Resolves Repeat-Rich Silk Genes.
Genome Biol Evol
; 16(7)2024 Jul 03.
Article
em En
| MEDLINE
| ID: mdl-38957923
ABSTRACT
We present the first long-read de novo assembly and annotation of the luna moth (Actias luna) and provide the full characterization of heavy chain fibroin (h-fibroin), a long and highly repetitive gene (>20 kb) essential in silk fiber production. There are >160,000 described species of moths and butterflies (Lepidoptera), but only within the last 5 years have we begun to recover high-quality annotated whole genomes across the order that capture h-fibroin. Using PacBio HiFi reads, we produce the first high-quality long-read reference genome for this species. The assembled genome has a length of 532 Mb, a contig N50 of 16.8 Mb, an L50 of 14 contigs, and 99.4% completeness (BUSCO). Our annotation using Bombyx mori protein and A. luna RNAseq evidence captured a total of 20,866 genes at 98.9% completeness with 10,267 functionally annotated proteins and a full-length h-fibroin annotation of 2,679 amino acid residues.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Genoma de Inseto
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Anotação de Sequência Molecular
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Fibroínas
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Mariposas
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article