High-throughput G protein-coupled receptor-based autocrine screening for secondary metabolite production in yeast.
Biotechnol Bioeng
; 121(10): 3283-3296, 2024 Oct.
Article
em En
| MEDLINE
| ID: mdl-38973176
ABSTRACT
Biosensors are valuable tools in accelerating the test phase of the design-build-test-learn cycle of cell factory development, as well as in bioprocess monitoring and control. G protein-coupled receptor (GPCR)-based biosensors enable cells to sense a wide array of molecules and environmental conditions in a specific manner. Due to the extracellular nature of their sensing, GPCR-based biosensors require compartmentalization of distinct genotypes when screening production levels of a strain library to ensure that detected levels originate exclusively from the strain under assessment. Here, we explore the integration of production and sensing modalities into a single Saccharomyces cerevisiae strain and compartmentalization using three different methods:
(1) cultivation in microtiter plates, (2) spatial separation on agar plates, and (3) encapsulation in water-in-oil-in-water double emulsion droplets, combined with analysis and sorting via a fluorescence-activated cell sorting machine. Employing tryptamine and serotonin as proof-of-concept target molecules, we optimize biosensing conditions and demonstrate the ability of the autocrine screening method to enrich for high producers, showing the enrichment of a serotonin-producing strain over a nonproducing strain. These findings illustrate a workflow that can be adapted to screening for a wide range of complex chemistry at high throughput using commercially available microfluidic systems.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Técnicas Biossensoriais
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Receptores Acoplados a Proteínas G
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Ensaios de Triagem em Larga Escala
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article