Association of Long Non-Coding RNA Malat1 with Serum Levels of Interleukin-1 Beta and Vitamin D in Patients with Ischemic Stroke.
Galen Med J
; 12: 1-10, 2023.
Article
em En
| MEDLINE
| ID: mdl-38974129
ABSTRACT
BACKGROUND:
Previous studies have demonstrated the strong association of inflammatory cytokines and vitamin D (VitD) deficiency and ischemic stroke (IS) pathogenesis. Due to the negative correlation between long non-coding RNA (lncRNA) Malat1 and pro-inflammatory factors we decided to investigate the associations between Malat1 expression with serum interleukin-1ß (IL-1ß), and VitD levels in IS patients. MATERIALS ANDMETHODS:
In this cross-sectional study, 63 IS patients were included. We used enzyme-linked immunosorbent assays to evaluate the serum levels of VitD and IL-1ß. Malat1 expression was evaluated by the real-time polymerase chain reaction test. The associations between Malat1expression with VitD and IL-1ß were analysed with linear regression (Stepwise model) and Pearson's correlation analysis.RESULTS:
The Malat1 expression was inversely correlated with stroke severity (r=-0.25, P=0.043). Stepwise regression analysis showed a significant positive relationship between VitD level and Malat1 expression (Beta=0.28, P=0.02), and also showed a non-significant negative relationship between IL-1ß and stroke severity. VitD level showed a positive Pearson correlation with Malat1 (r=0.28, P=0.023) and a negative correlation with IL-1ß (r=-0.29, P=0.018) while it could not detect a significantly negative correlation with stroke severity.CONCLUSION:
For the first time the associations between Malat1 expression with IL-1ß and VitD in IS patients was analyzed. We found a significant positive relationship between VitD and Malat1. This correlation needs to be investigated with a larger sample size to achieve a strong and reliable association between VitD and Malat1.
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MEDLINE
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En
Ano de publicação:
2023
Tipo de documento:
Article