Post-Integrational DNA Repair of HIV-1 Is Associated with Activation of the DNA-PK and ATM Cellular Protein Kinases and Phosphorylation of Their Targets.
Biochemistry (Mosc)
; 89(6): 1122-1132, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38981705
ABSTRACT
Integration of the DNA copy of HIV-1 genome into the cellular genome results in series of damages, repair of which is critical for successful replication of the virus. We have previously demonstrated that the ATM and DNA-PK kinases, normally responsible for repairing double-strand breaks in the cellular DNA, are required to initiate the HIV-1 DNA postintegrational repair, even though integration does not result in DNA double-strand breaks. In this study, we analyzed changes in phosphorylation status of ATM (pSer1981), DNA-PK (pSer2056), and their related kinase ATR (pSer428), as well as their targets Chk1 (pSer345), Chk2 (pThr68), H2AX (pSer139), and p53 (pSer15) during the HIV-1 DNA postintegrational repair. We have shown that ATM and DNA-PK, but not ATR, undergo autophosphorylation during postintegrational DNA repair and phosphorylate their target proteins Chk2 and H2AX. These data indicate common signaling mechanisms between the double-strand DNA break repair and postintegrational repair of HIV-1 DNA.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
HIV-1
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Reparo do DNA
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Proteína Quinase Ativada por DNA
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Proteínas Mutadas de Ataxia Telangiectasia
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article