Modulation of Bronchial Epithelial Barrier Integrity by Low Molecular Weight Components from Birch Pollen.
Int J Mol Sci
; 25(13)2024 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-39000481
ABSTRACT
Pollen, in addition to allergens, comprise low molecular weight components (LMC) smaller than 3 kDa. Emerging evidence indicates the relevance of LMC in allergic immune responses. However, the interaction of birch pollen (BP)-derived LMC and epithelial cells has not been extensively studied. We investigated epithelial barrier modifications induced by exposure to BP LMC, using the human bronchial epithelial cell line 16HBE14o-. Epithelial cell monolayers were apically exposed to the major BP allergen Bet v 1, aqueous BP extract or BP-derived LMC. Barrier integrity after the treatments was monitored by measuring transepithelial electrical resistance at regular intervals and by using the xCELLigence Real-Time Cell Analysis system. The polarized release of cytokines 24 h following treatment was measured using a multiplex immunoassay. Epithelial barrier integrity was significantly enhanced upon exposure to BP LMC. Moreover, BP LMC induced the repair of papain-mediated epithelial barrier damage. The apical release of CCL5 and TNF-α was significantly reduced after exposure to BP LMC, while the basolateral release of IL-6 significantly increased. In conclusion, the results of our study demonstrate that BP-derived LMC modify the physical and immunological properties of bronchial epithelial cells and thus regulate airway epithelial barrier responses.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pólen
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Brônquios
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Betula
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Células Epiteliais
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Peso Molecular
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article