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Electrospun Composites of Chitosan with Cerium Oxide Nanoparticles for Wound Healing Applications: Characterization and Biocompatibility Evaluation In Vitro and In Vivo.
Petrova, Valentina A; Poshina, Daria N; Golovkin, Alexey S; Mishanin, Alexander I; Zhuravskii, Sergei G; Yukina, Galina Y; Naumenko, Maria Y; Sukhorukova, Elena G; Savin, Nikita A; Erofeev, Alexander S; Gofman, Iosif V; Ivan'kova, Elena M; Dubashynskaya, Natallia V; Yakimansky, Alexander V; Skorik, Yury A.
Afiliação
  • Petrova VA; Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi VO 31, 199004 St. Petersburg, Russia.
  • Poshina DN; Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi VO 31, 199004 St. Petersburg, Russia.
  • Golovkin AS; Almazov National Medical Research Centre, Akkuratova 2, 197341 St. Petersburg, Russia.
  • Mishanin AI; Almazov National Medical Research Centre, Akkuratova 2, 197341 St. Petersburg, Russia.
  • Zhuravskii SG; Hearing and Speech Laboratory, Pavlov First Saint Petersburg State Medical University, L'va Tolstogo 6-8, 197022 St. Petersburg, Russia.
  • Yukina GY; Laboratory of Pathomorphology, Pavlov First Saint Petersburg State Medical University, L'va Tolstogo 6-8, 197022 St. Petersburg, Russia.
  • Naumenko MY; Hearing and Speech Laboratory, Pavlov First Saint Petersburg State Medical University, L'va Tolstogo 6-8, 197022 St. Petersburg, Russia.
  • Sukhorukova EG; Laboratory of Pathomorphology, Pavlov First Saint Petersburg State Medical University, L'va Tolstogo 6-8, 197022 St. Petersburg, Russia.
  • Savin NA; Laboratory of Biophysics, National University of Science and Technology "MISIS", Leninsky 4, 119049 Moscow, Russia.
  • Erofeev AS; Laboratory of Biophysics, National University of Science and Technology "MISIS", Leninsky 4, 119049 Moscow, Russia.
  • Gofman IV; Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi VO 31, 199004 St. Petersburg, Russia.
  • Ivan'kova EM; Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi VO 31, 199004 St. Petersburg, Russia.
  • Dubashynskaya NV; Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi VO 31, 199004 St. Petersburg, Russia.
  • Yakimansky AV; Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi VO 31, 199004 St. Petersburg, Russia.
  • Skorik YA; Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi VO 31, 199004 St. Petersburg, Russia.
Polymers (Basel) ; 16(13)2024 Jun 25.
Article em En | MEDLINE | ID: mdl-39000644
ABSTRACT
Cerium oxide nanoparticles (CeONPs), as part of tissue regeneration matrices, can protect cells from reactive oxygen species and oxidative stress. In addition, they can influence the properties of the scaffold, including its electrospinnability and mechanical strength. In this work, we prepared electrospun fiber mats from a chitosan and polyethylene oxide blend (CS-PEO) with the addition of ceria nanoparticles (CS-PEO-CeONP). The addition of CeONPs resulted in a smaller fiber diameter and higher swelling compared to CS-PEO fiber mats. CeONP-modified fiber mats also had a higher Young's modulus due to the reinforcing effect of the nanoparticles. Both mats had comparable adhesion and cytocompatibility to mesenchymal stem cells, which had a more rounded morphology on CS-PEO-CeONP compared to elongated cells on the CS-PEO mats. Biocompatibility in an in vivo rat model showed no acute toxicity, no septic or allergic inflammation, and no rough scar tissue formation. The degradation of both mats passed the stage of matrix swelling. CS-PEO-CeONP showed significantly slower biodegradation, with most of the matrix remaining in the tissue after 90 days. The reactive inflammation was aseptic in nature with the involvement of multinucleated foreign-body type giant cells and was significantly reduced by day 90. CeONPs induced the formation of the implant's connective tissue capsule. Thus, the introduction of CeONPs influenced the physicochemical properties and biological activity of CS-PEO nanofiber mats.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article