Protocol for real-time measurement of mitochondrial respiration in the mouse ocular posterior pole using a Seahorse XFe24 analyzer.
STAR Protoc
; 5(3): 103150, 2024 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-39002132
ABSTRACT
During aging and in retinal degenerative diseases, vulnerable retinal pigment epithelial (RPE) cells are subject to mitochondrial dysfunction, creating a need for accessibility to tools which can facilitate assessment of the ocular posterior pole bioenergetics. Here, we present a protocol for quantifying mitochondrial respiration in the posterior eye cup (RPE-choroid-sclera) of young and old mice. We describe steps for eye cup dissection, optimization of tissue size, drug concentrations, and cycle conditions using the XF Cell Mito Stress Test.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Epitélio Pigmentado da Retina
/
Mitocôndrias
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article