A flexible glucose biosensor modified by reduced-swelling and conductive zwitterionic hydrogel enzyme membrane.
Anal Bioanal Chem
; 416(22): 4849-4860, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-39008068
ABSTRACT
This paper reports a flexible glucose biosensor which is modified by a reduced-swelling and conductive zwitterionic hydrogel enzyme membrane that contains two forms of chemical cross-links. One chemical cross-linking is induced by thermal initiators and forms the basal network of the hydrogel. Another cross-linking is achieved by the coordination interactions between the multivalent metal ion Al3+ and anionic group -COO- of zwitterionic poly-carboxy betaine (pCBMA), which significantly increase the cross-linking density of the zwitterionic hydrogel, improving the reduced-swelling property and reducing the pore size. The better reduced-swelling property and reduced diameters of pores within the zwitterionic hydrogel make less glucose oxidase (GOx) leakage, thus significantly improving the enzyme membrane's service life. By introducing the Al3+ and Cl-, the conductivity of the zwitterionic hydrogel is enhanced approximately 10.4-fold. According to the enhanced conductivity, the reduced-swelling property, and the high GOx loading capacity of the zwitterionic hydrogel, the sensitivity of the biosensor with GOx/pCBMA-Al3+ is significantly improved by 5 times and has a long service life. Finally, the proposed GOx/pCBMA-Al3+ biosensor was applied in non-invasive blood glucose detection on the human body, verifying the capability in practice.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Condutividade Elétrica
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Enzimas Imobilizadas
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Glucose
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Glucose Oxidase
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article