Metabolic dysfunctions in pearl oysters following recurrent marine heatwaves.

ABSTRACT

Marine heatwaves (MHWs) have become more frequent, intense and extreme in oceanic systems in the past decade, resulting in mass mortality events of marine invertebrates and devastating coastal marine ecosystems. While metabolic homeostasis is a fundamental requirement in stress tolerance, little is known about its role under intensifying MHWs conditions. Here, we investigated impacts of MHWs on the metabolism in pearl oysters (Pinctada maxima) - an ecologically and economically significant bivalve species in tropical ecosystems. Activities of digestive enzymes (gastric proteases, lipases, and amylases) did not significantly respond to various scenario of recurrent MHWs varying from 24 °C to 28 °C (moderate) and 32 °C (severe). The metabolomics analysis revealed nine and five key metabolism pathways under both MHWs scenarios. Specifically, pathways associated with energy metabolism were impaired by moderate MHWs, manifesting in downregulation of differential metabolite (The nicotinic acid and N-acetyl-glutamic acid). The content of CDP-ethanolamine was significantly decrease, and the perturbations of oxidative stress caused by the decreased of content of D-glutamine. Metabolites related to a suite of body functions (e.g., the lipid metabolism, biomineralization, and antioxidant defenses) showed significantly negative responses by severe MHWs. These findings reveal the metabolic impairments of marine bivalves when subjected to MHWs varying in intensity and frequency, implying cascading consequences which deserve further investigation.