Crofton weed derived isomers of ageraphorone as potent antifeedant against Plutella xylostella (L.).
Ecotoxicol Environ Saf
; 282: 116729, 2024 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-39024945
ABSTRACT
Global agricultural production is significantly hampered by insect pests, and the demand for natural pragmatic pesticides with environmental concern remains unfulfilled. Ageratina adenophora (Spreng.) also known as Crofton weed, is an invasive perennial herbaceous plant that is known to possess multiple bioactive compounds. In our study, two isomers of ageraphorone metabolites i.e, 10â¯Hα-9-oxo-ageraphorone (10HA) and 10â¯Hß-9-oxo-ageraphorone (10HB), were identified from Crofton weed, exhibiting potent antifeedant and larvicidal activities against Plutella xylostella. For antifeedant activity, the median effective concentration (EC50) values for 10HA and 10HB in the choice method were 2279â¯mg/L and 3233â¯mg/L, respectively, and for the no choice method, EC50 values were 1721â¯mg/L and 2394â¯mg/L, respectively. For larvicidal activity, lethal concentration (LC50) values for 10HA and 10HB were 2421â¯mg/L and 4109â¯mg/L at 48â¯h and 2101â¯mg/L and 3550â¯mg/L at 72â¯h. Furthermore, both in- vivo and in-vitro studies revealed that the isomers 10HA and 10HB exhibited potent detoxifying enzymes inhibition activity such as carboxylesterase and glutathione S-transferases. Molecular docking and MD simulation analysis provide insight into the possible interaction between isomers of ageraphorone metabolites and Carboxylic Ester Hydrolase protein (Gene pxCCE016b) of P. xylostella, which led to a finding that CarEH protein plays a significant role in the detoxification of the two compounds in P. xylostella. Finally, our findings show that the primary enzymes undergoing inhibition by isomers of ageraphorone metabolites, causing toxicity in insects, are Carboxylesterase and glutathione S-transferase.
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Base de dados:
MEDLINE
Assunto principal:
Sesquiterpenos
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Ageratina
/
Mariposas
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article