Time-Resolved Ion Mobility-Mass Spectrometry Reveals Structural Transitions in the Disassembly of Modular Polyketide Syntheses.
J Am Soc Mass Spectrom
; 35(9): 2136-2142, 2024 Sep 04.
Article
em En
| MEDLINE
| ID: mdl-39038158
ABSTRACT
The type 1 polyketide synthase (PKS) assembly line uses its modular structure to produce polyketide natural products that form the basis of many pharmaceuticals. Currently, several cryoelectron microscopy (cryo-EM) structures of a multidomain PKS module have been constructed, but much remains to be learned. Here we utilize ion-mobility mass spectrometry (IM-MS) to record size and shape information and detect different conformational states of a 207 kDa didomain dimer comprised of ketosynthase (KS) and acyl transferase (AT), excised from full-length module. Furthermore, gas-phase stability differences between these different conformations are captured by collision induced unfolding (CIU) technology. Additionally, through tracking these forms as a function of time, we elucidate a detailed disassembly pathway for KS-AT dimers for the first time.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Policetídeo Sintases
/
Policetídeos
/
Espectrometria de Mobilidade Iônica
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article