The Structural Characterization of a Polysaccharide from the Dried Root of Salvia miltiorrhiza and Its Use as a Vaccine Adjuvant to Induce Humoral and Cellular Immune Responses.
Int J Mol Sci
; 25(14)2024 Jul 16.
Article
em En
| MEDLINE
| ID: mdl-39063007
ABSTRACT
In order to supplement the research gap concerning Salvia miltiorrhiza polysaccharide extracted from Danshen in NMR analysis, and to clarify its immune enhancement effect as an adjuvant, we isolated and purified SMPD-2, which is composed of nine monosaccharides such as Ara, Gal, and Glc from Danshen. Its weight average molecular weight was 37.30 ± 0.096 KDa. The main chain was mainly composed of â4)-α-D-Galp-(1â, â3,6)-ß-D-Glcp-(1â and a small amount of α-L-Araf-(1â. After the subcutaneous injection of SMPD-2 as an adjuvant to OVA in mice, we found that it enhanced the immune response by activating DCs from lymph nodes, increasing OVA-specific antibody secretion, stimulating spleen lymphocyte activation, and showing good biosafety. In conclusion, SMPD-2 could be a promising candidate for an adjuvant.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Adjuvantes Imunológicos
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Raízes de Plantas
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Salvia miltiorrhiza
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Imunidade Humoral
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Imunidade Celular
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article