Effect of 17ß-Estradiol on Endothelial Cell Expression of Inflammation-Related MicroRNA.

Levy, Ma'ayan V; Fandl, Hannah K; Hijmans, Jamie G; Stockelman, Kelly A; Ruzzene, Samuel T; Reiakvam, Whitney R; Goldthwaite, Zoe A; Greiner, Jared J; DeSouza, Christopher A; Garcia, Vinicius P

Levy, Ma'ayan V; Fandl, Hannah K; Hijmans, Jamie G; Stockelman, Kelly A; Ruzzene, Samuel T; Reiakvam, Whitney R; Goldthwaite, Zoe A; Greiner, Jared J; DeSouza, Christopher A; Garcia, Vinicius P.

Afiliação

Levy MV; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
Fandl HK; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
Hijmans JG; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
Stockelman KA; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
Ruzzene ST; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
Reiakvam WR; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
Goldthwaite ZA; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
Greiner JJ; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
DeSouza CA; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.
Garcia VP; Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado Boulder, Colorado 80309, USA.

Microrna ; 2024 Jul 25.

Article em En | MEDLINE | ID: mdl-39069709

ABSTRACT

ABSTRACT

Introduction/

Objective:

Estrogen plays a protective role in vascular health due, in part, to its regulation of endothelial inflammation. However, the mechanism(s) by which estrogen negatively regulates inflammatory signaling pathways is not completely understood. MicroRNAs (miRNAs) are recognized as sensitive and selective regulators of cardiovascular function, inflammation, and disease, yet the effects of 17ß-estradiol on the endothelial miRNA profile are largely unknown. The aim of this study was to determine the effect of 17ß-estradiol on the expression of inflammation-associated miRNAs in endothelial cells in vitro.

METHODS:

Human Umbilical Vein Endothelial cells (HUVECs) were treated with media in the absence (control) and presence of 17ß-estradiol (100 nM) for 24 hr. Thereafter, endothelial cell release of cytokines (IL-6 and IL-8), the intracellular expression of the central protein inflammatory mediator NF- B, and the levels of inflammatory-associated miRNAs miR-126, miR-146a, miR-181b, miR-204, and miR-let-7a, were determined.

RESULTS:

17ß-estradiol-treated cells released significantly lower levels of IL-6 (47.6±1.5 pg/mL vs 59.3±4.9 pg/mL) and IL-8 (36.3±2.3 pg/mL vs 44.0±2.0 pg/mL). Cellular expression of total NF- B (26.0±2.8 AU vs 21.2±3.1 AU) was not different between groups; however, activated NF- B (Ser536) (12.9±1.7 AU vs 20.2±2.2 AU) was markedly reduced in 17ß-estradiol-treated cells as compared to untreated cells. Furthermore, cellular expressions of miR-126 (1.8±0.3 fold), miR-146a (1.7±0.3 fold), miR-181b (2.1±0.4 fold), miR-204 (1.9±0.4 fold), and miR-Let-7a (1.8±0.3 fold) were markedly increased in response to 17ß-estradiol treatment.