Protocol for the isolation and purification of endoplasmic reticulum-plasma membrane junctions from the mouse brain.
STAR Protoc
; 5(3): 103253, 2024 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-39126654
ABSTRACT
Dynamic communication between intracellular organelles often takes place at specialized membrane contact sites that form between their membranes. Here we detail a procedure for the purification of endoplasmic reticulum-plasma membrane (ER-PM) junctions from the mouse brain. We describe steps for homogenizing isolated brain hemispheres and sequential centrifugation to remove the nuclear fraction from the other membrane fractions. We then detail procedures for separating the resulting crude membrane fractions by sucrose density gradients and purifying into their respective pellets. For complete details on the use and execution of this protocol, please refer to Weesner et al.1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Fracionamento Celular
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Membrana Celular
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Retículo Endoplasmático
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article