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Laccase Immobilized on Arginine-Functionalized Boron Nitride Nanosheets for Enhanced Atrazine Degradation.
Gao, Yifan; Xiao, Minhao; Zou, Haiyuan; Nurwono, Glenn; Zgonc, David; Birch, Quinn; Nadagouda, Mallikarjuna N; Park, Junyoung O; Blotevogel, Jens; Liu, Chong; Hoek, Eric M V; Mahendra, Shaily.
Afiliação
  • Gao Y; Department of Civil and Environmental Engineering, University of California, Los Angeles, 580 Portola Plaza, Los Angeles, California 90095, United States.
  • Xiao M; Department of Civil and Environmental Engineering, University of California, Los Angeles, 580 Portola Plaza, Los Angeles, California 90095, United States.
  • Zou H; Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive East, Los Angeles, California 90095, United States.
  • Nurwono G; Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive East, Los Angeles, California 90095, United States.
  • Zgonc D; Department of Civil and Environmental Engineering, University of California, Los Angeles, 580 Portola Plaza, Los Angeles, California 90095, United States.
  • Birch Q; Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, Ohio 45221, United States.
  • Nadagouda MN; Office of Research & Development Center for Environmental Solutions & Emergency Response, United States Environmental Protection Agency, 26 W. Martin Luther King Drive, Cincinnati, Ohio 45268, United States.
  • Park JO; Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, Los Angeles, California 90095, United States.
  • Blotevogel J; CSIRO Environment, Waite Campus, Urrbrae, SA 5064, Australia.
  • Liu C; Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive East, Los Angeles, California 90095, United States.
  • Hoek EMV; Department of Civil and Environmental Engineering, University of California, Los Angeles, 580 Portola Plaza, Los Angeles, California 90095, United States.
  • Mahendra S; Department of Civil and Environmental Engineering, University of California, Los Angeles, 580 Portola Plaza, Los Angeles, California 90095, United States.
Environ Sci Technol ; 2024 Aug 12.
Article em En | MEDLINE | ID: mdl-39132890
ABSTRACT
Enzyme-mediated systems have been widely employed for the biotransformation of environmental contaminants. However, the catalytic performance of free enzymes is restricted by the rapid loss of their catalytic activity, stability, and reusability. In this work, we developed an enzyme immobilization platform by elaborately anchoring fungal laccase onto arginine-functionalized boron nitride nanosheets (BNNS-Arg@Lac). BNNS-Arg@Lac showcased ∼75% immobilization yield and enhanced stability against fluctuating pH values and temperatures, along with remarkable reusability across six consecutive cycles, outperforming free natural laccase (nlaccase). A model pollutant, atrazine, was selected for a proof-of-concept demonstration, given the substantial environmental and public health concerns in agriculture runoff. BNNS-Arg@Lac-catalyzed atrazine degradation rate was nearly twice that of nlaccase. Moreover, BNNS-Arg@Lac consistently demonstrated superior atrazine degradation in synthetic agricultural wastewater and various mediator systems compared to nlaccase. Comprehensive product analysis unraveled distinct degradation pathways for BNNS-Arg@Lac and nlaccase. Overall, this research provides a foundation for the future development of enzyme-nanomaterial hybrids for degrading environmental chemicals and may unlock new potential for green and efficient resource recovery and waste management strategies.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article