AcsS Negatively Regulates the Transcription of type VI Secretion System 2 Genes in Vibrio parahaemolyticus.
Curr Microbiol
; 81(10): 330, 2024 Aug 28.
Article
em En
| MEDLINE
| ID: mdl-39196442
ABSTRACT
The type VI secretion system 2 (T6SS2) gene cluster of Vibrio parahaemolyticus comprises three operons VPA1027-1024, VPA1043-1028, and VPA1044-1046. AcsS is a LysR-like transcriptional regulator that play a role in activating flagella-driven motility in V. parahaemolyticus. However, its potential roles in other cellular pathways remain poorly understood. In this study, we conducted a series of experiments to investigate the regulatory effects of AcsS on the transcription of VPA1027 (hcp2), VPA1043, and VPA1044. The findings revealed that AcsS indirectly inhibits the transcription of these genes. Additionally, deletion of acsS resulted in enhanced adhesion of V. parahaemolyticus to HeLa cells. However, disruption of T6SS2 alone or in conjunction with AcsS significantly diminished the adhesion capacity of V. parahaemolyticus to HeLa cells. Therefore, it is suggested that AcsS suppresses cell adhesion in V. parahaemolyticus by downregulating the transcription of T6SS2 genes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Transcrição Gênica
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Vibrio parahaemolyticus
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Aderência Bacteriana
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Regulação Bacteriana da Expressão Gênica
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Sistemas de Secreção Tipo VI
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article