Two paralogous PHD finger proteins participate in natural genome editing in Paramecium tetraurelia.
J Cell Sci
; 137(16)2024 08 15.
Article
em En
| MEDLINE
| ID: mdl-39212120
ABSTRACT
The unicellular eukaryote Paramecium tetraurelia contains functionally distinct nuclei germline micronuclei (MICs) and a somatic macronucleus (MAC). During sex, the MIC genome is reorganized into a new MAC genome and the old MAC is lost. Almost 45,000 unique internal eliminated sequences (IESs) distributed throughout the genome require precise excision to guarantee a functional new MAC genome. Here, we characterize a pair of paralogous PHD finger proteins involved in DNA elimination. DevPF1, the early-expressed paralog, is present in only some of the gametic and post-zygotic nuclei during meiosis. Both DevPF1 and DevPF2 localize in the new developing MACs, where IES excision occurs. Upon DevPF2 knockdown (KD), long IESs are preferentially retained and late-expressed small RNAs decrease; no length preference for retained IESs was observed in DevPF1-KD and development-specific small RNAs were abolished. The expression of at least two genes from the new MAC with roles in genome reorganization seems to be influenced by DevPF1- and DevPF2-KD. Thus, both PHD fingers are crucial for new MAC genome development, with distinct functions, potentially via regulation of non-coding and coding transcription in the MICs and new MACs.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Protozoários
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Paramecium tetraurellia
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Edição de Genes
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article